{"id":6692,"date":"2025-09-09T11:36:53","date_gmt":"2025-09-09T08:36:53","guid":{"rendered":"https:\/\/kbfi.ee\/?page_id=6692"},"modified":"2025-09-10T14:39:48","modified_gmt":"2025-09-10T11:39:48","slug":"services","status":"publish","type":"page","link":"https:\/\/kbfi.ee\/en\/cb\/services\/","title":{"rendered":"SERVICES"},"content":{"rendered":"<p>The CB laboratory utilizes <a href=\"https:\/\/www.bioblast.at\/index.php\/High-resolution_respirometry\"><strong>high-resolution respirometry<\/strong><\/a>, a modern oxygraphy method, to precisely measure oxygen consumption, directly reflecting mitochondrial respiratory activity in samples. By applying specific substrates and inhibitors, this method allows assessment of mitochondrial integrity and the activity of distinct mitochondrial functions, including electron transport chain complexes.<\/p>\n<p>The CB laboratory has expertise in handling a wide range of human cell lines. A l<strong>aminar-flow oxygen chamber<\/strong> enables experiments under controlled oxygen concentrations, including prolonged culture at 5% oxygen, corresponding to physiological tissue levels (physioxia).<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Using the competence and methods mentioned above, the Laboratory of Chemical Biology offers the following services:<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Functional assessment of muscle performance and recovery capacity<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Functional assessment of muscle performance and recovery capacity can be conducted on a muscle biopsy using high-resolution respirometry, which enables the evaluation of overall mitochondrial energy-productionrate, the activity of individual respiratory chain complexes in mitochondria, as well as the type and efficiency of energy transfer (creatine kinase and adenylate kinase pathway activities). This approach allows the identification of potential primary causes underlying impaired muscle function and permits the monitoring of therapeutic efficacy. Furthermore, the methodology is applicable in animal models across a range of disease conditions and in initial \u00a0stage of pharmacological research.<\/p>\n<ul>\n<li>Method \u2013 high resolution respirometry<\/li>\n<li>Model \u2013 muscle biopsy<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Determination of drug and drug-carrier movement into and within the cell.<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Our laboratory is equipped to determine the intracellular transfer of drugs and drug carriers, enabling analysis of their localization within specific regions of both fixed and live cells. Additionally, we \u00a0can conduct preliminary functional studies to evaluate the impact of the compounds on cellular metabolism.<\/p>\n<ul>\n<li>Method \u2013 confocal microscopy, high resolution respirometry<\/li>\n<li>Model \u2013 cell culture, tissue samples, animal model<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Assessment of the effects and side effects of drug candidates on cellular energy metabolism. <\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>We determine the impact of drugs or drug carriers on cellular (energy) metabolism, providing rapid results that inform decision-making before\u00a0 progression into large-scale studies.<\/p>\n<ul>\n<li>Method \u2013 confocal microscopy, high resolution respirometry<\/li>\n<li>Model \u2013 cell culture, tissue samples, animal model<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Evaluation of the toxicity of chemicals and pesticides on cell cultures.<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Using the methodologies available in our laboratory, it is possible to evaluate both cell viability and subtle functional alterations in cellular energy metabolism that precede significant declines in viability.<\/p>\n<ul>\n<li>Method \u2013 high resolution respirometry<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Impact of environmental changes on cellular energy metabolism.<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Comprehensive analyses can be performed to determine metabolic alterations in cells exposed to unfavorable (e.g., polluted) environments. In addition, the effects of contaminants on tissue energy metabolism can be tested using animal models. Aquatic organisms can be assessed as well, enabling the identification of pathological processes in energy metabolism associated with such exposures.<\/p>\n<ul>\n<li>Method \u2013 high resolution respirometry<\/li>\n<li>Model \u2013 cell culture, tissue samples from animal model including aquatic organisms.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Assessment of the impact of extracellular vesicle\u2013mediated cargo on cellular energy metabolism.<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Studies have demonstrated that extracellular vesicles influence cellular metabolism by transferring bioactive molecules, including proteins, peptide fragments, and other biomolecules, which then modulate the function of the recipient cell. \u00a0Using high resolution respirometry as a rapid functional assay, the effects of extracellular vesicles on cellular metabolism, with a particular focus on energy metabolism, can be evaluated.<\/p>\n<ul>\n<li>Method \u2013 high resolution respirometry<\/li>\n<li>Model \u2013 cell culture, animal model<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>\n<h4><span style=\"color: #993366;\"><strong>Assessment of changes in blood cells energy metabolism in pathology.<\/strong><\/span><\/h4>\n<\/li>\n<\/ul>\n<p>Using cellular blood components, such as platelets, our laboratory can rapidly detect alterations in energy metabolism, providing quick functional insights before progression to large-scale studies.<\/p>\n<ul>\n<li>Method \u2013 high resolution respirometry<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>If you would like to collaborate or have any questions, please don&#8217;t hesitate to contact us:<\/strong><\/p>\n<ul>\n<li><strong>Kersti Tepp<\/strong> kersti.tepp@kbfi.ee<\/li>\n<li><strong>Tuuli K\u00e4\u00e4mbre<\/strong> tuuli.kaambre@kbfi.ee<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The CB laboratory utilizes high-resolution respirometry, a modern oxygraphy method, to precisely measure oxygen consumption, directly reflecting mitochondrial respiratory activity in samples. By applying specific substrates and <a class=\"read-more\" href=\"https:\/\/kbfi.ee\/en\/cb\/services\/\"><\/a><\/p>\n","protected":false},"author":5,"featured_media":0,"parent":102,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-6692","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/pages\/6692"}],"collection":[{"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/comments?post=6692"}],"version-history":[{"count":0,"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/pages\/6692\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/pages\/102"}],"wp:attachment":[{"href":"https:\/\/kbfi.ee\/en\/wp-json\/wp\/v2\/media?parent=6692"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}